The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy

• Yao Yao,
• Yeongun Ko,
• Grant Grasman,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30

• be reached, the identification of structure–function and material–biological relations of NPs could be dramatically accelerated. Prevalence of reporting imaging and (or) flow cytometry techniques, protein corona, 3D cell culture model(s), and serum content during nanoparticle incubation with cells in

• investigating NP cellular uptake and (or) transfection. (c) 5-year prevalence of describing or characterizing protein corona in the manuscript. (d) 5-year prevalence of employing 3D cell culture models (e.g., spheroids or organoids). (e) Comparisons of serum content used during nanoparticle incubation with

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• ; mesenchymal stem cells; quantum dots; spheroids; 3D cell culture; Introduction The recent progress in the development of nanoscale agents opens up new perspectives for targeted drug delivery in cancer diagnostics, imaging and therapy. However, once administered into the body, nanoparticles (NPs) are rapidly

• uptake pathway in 3D conditions likely due to poor inhibitor penetrance into the spheroids. In general, 3D cultures are extensively studied given their potential to mimic cellular interactions and signal transduction occurring in in vivo conditions [14]. Briefly, 3D cell culture formation occurs on

• up to 90% confluence in complete cell culture medium in a humidified chamber at 37 °C with 5% CO2. For passaging, the cells were trypsinised using 0.25% trypsin–EDTA solution. All cell reagents were purchased from Sigma-Aldrich, St. Louis, MO, USA. Establishment of 3D cell culture model A poly(2

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• of the human airway/alveolar epithelial barrier. The human 3D cell culture model consists of three cell types, i.e., alveolar epithelial cells, macrophages, and dendritic cells as described in [127]. To imitate the lung organ structure, an A549 cell layer was cultured on porous membranes with human